Iwashita M, Watanabe M, Ishii M, Chen T, Johnson SL, Kurachi Y, Okada…, 2006 · passages 30 to 32 of 33

Pigment pattern in jaguar/obelix zebrafish is caused by a Kir7.1 mutation: implications for the regulation of melanosome movement

Measurement of melanophore response to α2-adrenoceptor agonist and antagonist.

Likewise, to measure the α2-adrenoceptor dispersion response to the antagonist yohimbine (Sigma, http://www.signaaldrich.com), a white background color was maintained to block endogenous dispersion. We observed and scanned the response of the melanophore to yohimbine for a longer period (60 min) as the aggregation state was already induced by endogenous neurotransmitters that bind to α2-adrenoceptor tightly and because antagonist (yohimbine) competes with these neurotransmitters to bind the receptor. Yohimbine was added to the breeding tank to a final concentration of 10 μM.

Accession Numbers

The INSDC (International Nucleotide Sequence Database Collaboration; http://www.insdc.org) accession number reported in this paper for Kir7.1 is AB271018.

Acknowledgments

We thank the Tübingen Stock Centre (Tübingen, Germany), D. Parichy, for providing the mutant fish, M. Okamura and K. Bando for technical support, and H. Nojima for technical advice. We thank the anonymous reviewers for their helpful and in-depth comments.